774 TSG-6 and TSP-1 exhibit altered expression in the skin of diabetic patients

Diabetes Mellitus and chronic hyperglycemia are associated with many complications including delayed wound healing diabetic foot ulcers. Mechanisms behind this failure remain poorly understood but may include differential expression of two molecules, tumor necrosis factor stimulated gene 6 (TSG-6) thrombospondin 1 (TSP-1). These proteins, which interact each other, play important roles in display altered mouse models cell cultures. To our knowledge, the relationship between TSG-6 TSP1, diabetes, has never before been studied humans. Here, we undertook a prospective study non-diabetic patients wounds who were recruited from podiatry clinic. Tissue samples clinical data (i.e. HbA1c, size, demographics, confounding medical history) collected at visit. Western blot qPCR performed on tissue to assess levels TSG-6, TSP-1, pro- anti-inflammatory cytokines, leukocyte markers. Similar molecular also lower extremity amputations cross-sectional study. Skin normoglycemic (defined as HbA1c ≤ 6.4) had significantly TSP-1 than skin poor glycemic control (p=0.02). Conversely, control. We have demonstrated clear association regulate inflammation angiogenesis. This suggests new mechanism for impaired diabetes. Our translation conceptual paradigm mice humans increases its relevance offers potential predictive biomarkers biological targets future treatment non-healing wounds.